I-CPT tube 8ml

Incazelo emfushane:

Imodeli No.	I-CPT08
Okubalulekile	I-Neutral Pharmaceutical Glass
Isengezo	I-Anticoagulant + Gel + Ficoll Solution
Isicelo	Ukukhishwa kwe-Mononuclear Cell ukuze kwenziwe ucwaningo
Usayizi weThubhu	16*120mm
Dweba Ivolumu	8ML
Izinga lokuphepha	Izinga Lokuphepha Lamazwe Ngamazwe
Amayunithi Athengisa	200 amashubhu / ibhokisi
Umbala Wekhephu	Okumnyama & Okuluhlaza okwesibhakabhaka
Isampula	Iyatholakala
I-Shelf Life	Iminyaka engu-1
OEM/ODM	Ilebula, impahla, ukwakheka kwephakheji kuyatholakala.
Isitifiketi Sekhwalithi	ISO13485, FSC, MDSAP
Express	I-DHL, i-FedEx, i-TNT, i-UPS, i-EMS, i-SF, njll.
Inkokhelo	L/C, T/T, Western Union, Paypal, njll.

Ukupakisha

Iphakethe ngalinye esikhwameni sephepha;

Thumela i-imeyili kithi

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

I-Vacutainer CPT (Cell Preparation Tube) 8mL iyishubhu elikhethekile lokuqoqa igazi elisetshenziselwa ukuhlukanisa amaseli e-mononuclear (PBMCs) egazini lonke. Ivame ukusetshenziswa ku-immunology, ucwaningo lwezifo ezithathelwanayo, kanye nezicelo zomtholampilo ezifana nokuqapha amasosha omzimba kanye nokuhlolwa okusekelwe kumaseli.

Izici ezibalulekile ze-Vacutainer CPT Tube (8mL):
Ifakwe ngaphambili nge-anticoagulant kanye ne-density gradient medium
Iqukethe i-Sodium Citrate njenge-anticoagulant.
Kufaka ijeli yokuhlukanisa i-Ficoll™-based density gradient ukuze kube lula ukuhlukaniswa kwamaseli e-mononuclear.
Inika amandla ukuhlukaniswa kwe-PBMC okuyisinyathelo esisodwa
Iqeda isidingo se-centrifugation yendabuko yokuminyana kwe-gradient (isb, i-Ficoll-Paque™).
Yehlisa isikhathi sokucubungula nokuhlukahluka.
Umthamo wegazi ojwayelekile
Idizayinelwe ukucubungula kufika ku-8mL wegazi eliphelele.
Ishubhu eliyinyumba, elisetshenziswa kanye
Isetshenziselwa izicelo zomtholampilo nezocwaningo lapho ukuhlukaniswa kwe-PBMC kwekhwalithi ephezulu kuyadingeka.
Iyahambisana ne-centrifugation
Iphrothokholi ethile ye-centrifugation iyadingeka ukuze kuhlukaniswe ngempumelelo ama-PBMC kwezinye izingxenye zegazi.

Ukusetshenziswa kanye nezicelo:
Ukuhlukaniswa kwe-PBMC kwezifundo zokusebenza kwamasosha omzimba (amaseli T, amaseli B, amaseli e-NK, ama-monocyte).
I-Flow cytometry, i-ELISPOT, ukuhlolwa kwe-cytokine, nokunye ukuhlaziya kwe-immunological.
Ucwaningo lwezifo ezithathelwanayo nomgomo.
Ukuqapha izifo ze-autoimmune.
Izivivinyo zomtholampilo kanye nokutholwa kwe-biomarker.

Indlela Esebenza Ngayo (Inqubo Eyisisekelo):
1. Qoqa isampula yegazi kushubhu ye-CPT (gcwalisa ivolumu ebekiwe).
2. Guqula ngobumnene (izikhathi ezingu-8-10) ukuze uxube ne-anticoagulant.
3. I-Centrifuge ngokomthetho womkhiqizi ngamunye (imvamisa icishe ibe ngu-1500–1800 RCF imizuzu engama-20–30 ekamelweni lokushisa).
4. Amafomu ongqimba we-PBMC ngaphezu kwesihlukanisi sejeli ngemva kokuhlanganisa i-centrifugation.
5. Khipha ngokucophelela isendlalelo se-PBMC usebenzisa i-piette yezinhlelo zokusebenza ezansi nomfula.

Isitoreji Nokuphatha:
Gcina ekamelweni lokushisa (15-25 ° C).
Gwema ukushisa okudlulele noma ukubanda.